Hey,
I need to have Windows available, so I decided to install Windows 7 on my Mac. I recently upgraded to macOS 10.12 Sierra Public Beta (currently, I have PB 2), but I don't think that the problem is here because of the beta OS (I had the same issue few years ago on production version of OS).
I tried to run Boot Camp Assistant, but when I click Continue, message with text "Not enough space on startup disk to repartition. You must have at least 50GB of free space." (I have macOS in Czech, so I translated it), which is weird. I have 63GB free and I have SSD (128GB), so there shouldn't be any problem with fragmentation.
Can you help me with this? I tried everything, including disk repair in recovery mode.