Same problem ..
I'll see with my developper
did you try on https plateform? perhaps https is mandatory on iOs10?
Dr Charles Paoli
When logging the request from safari the server throws a 200. When quicktime opens the server throws a 401 ( Unauthorized) so it seems cookies for the domain are not send by quicktime on iOs 10
We are facing a similar issue. I filed a bug report with Apple about it at the time. It appears to be fixed in the iOS 10.2 betas. Not that it's a fix until that releases but perhaps see if it works there.